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Product Name
NSUN2 Polyclonal Antibody
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Description
Polyclonal antibody to NSUN2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
NSUN2 antibody; MISU antibody; MRT5 antibody; SAKI antibody; TRM4 antibody; tRNA (cytosine-C)-methyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 508-767 of human NSUN2 (NP_060225.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NSUN2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NSUN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunofluorescence - NSUN2 Polyclonal Antibody
Immunofluorescence analysis of C6 cells using NSUN2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - NSUN2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using NSUN2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.
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