NRC-16 peptide

NRC-16 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

1 Vial

GWKKWLRKGAKHLGQAAIK

Gly-Trp-Lys-Lys-Trp-Leu-Arg-Lys-Gly-Ala-Lys-His-Leu-Gly-Gln-Ala-Ala-Ile-Lys

19

96.9%

Synthetic

C-terminal NH2. Active against A. salmonicida 99-1, A. salmonicida 97-4, S. enterica serovar Typhimurium MS7953s (MIC 0.5-2 uM), S. enterica serovar Typhimurium 14028s (MIC 16 uM), P. aeruginosa K799, P. aeruginosa Z61, E. coli CGSC4908, E. coli UB1005 (MIC 1-4 uM), E. coli DC2 (MIC 0.5 uM), S. epidermidis C621(MIC 16 uM), MRSA C623 (MIC 32 uM), C. albicans C627 (MIC 8 uM). NRC-16 has Antibacterial, Antifungal activity. NRC-16 was found in witch flounder GC3.8-t, Glyptocephalus cynoglossus L..

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Patrzykat A, Gallant JW, Seo JK, Pytyck J, Douglas SE. Novel antimicrobial peptides derived from flatfish genes. Antimicrob Agents Chemother. 2003;47(8):2464-70.

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues