NOX1 Polyclonal Antibody

Cat.#: 161032

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Product Information

  • Product Name
    NOX1 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to NOX1
  • Tested applications
    WB
  • Species reactivity
    Mouse, Rat
  • Alternative names
    NOX1 antibody; GP91-2 antibody; MOX1 antibody; NOH-1 antibody; NOH1 antibody; NADPH oxidase 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NOX1 (NP_008983.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - NOX1 Polyclonal Antibody

    Western blot - NOX1 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using NOX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"