Nonaarginine/(Arg)9 peptide

Nonaarginine/(Arg)9 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RRRRRRRRR

H-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-OH

9

95.4%

C₅₄H₁₁₀N₃₆O₁₀

1423.7

2283335-13-5

Synthetic

Powder

Nonaarginine/(Arg)9 is a cell-penetrating peptide comprising 9 arginine residues capable of traversing plasma membranes. It has been shown that Arg9 destabilizes and disrupts the phospholipid bilayer allowing flow of ions across the membrane. (Arg)9 peptide plays an important role in efficient cellular uptake. The guanidinium group is a critical structural determinant for tight and rapid interactions with cell membranes. The cationic guanidinium group can form electrostatic interactions with anionic cell membrane components such as phospholipids and sulfated proteoglycans. This interaction can trigger the activation of specific intracellular signaling and cell internalization via various pathways. (Arg)9 peptide delivers a variety of functionally active cargos such as peptides, small interfering RNA, oligonucleotides, plasmid DNA, and liposomes into mammalian cells and plant cells with high translocation efficiency. (Arg)9 peptide can also form chemical linkages with the cargo and act as a carrier peptide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• J.P.Richard et al., J. Biol. Chem., 278, 585 (2003)
• S.M.Fuchs and R.T.Raines, Biochemistry, 43, 2438 (2004)
• R.Fischer et al., ChemBioChem, 7, 1428 (2006)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.