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Product Name
H1N1 Influenza NS1 (His Tag) recombinant protein
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Description
Inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism (By similarity).
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Protein name
Non-structural protein 1
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Uniprot ID
C8XP22
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Gene Name
NS1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) NS1 protein (C8XP22) (Asp 2-Val 230) was expressed, with a polyhistidine tag at the N-terminus.
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Protein Species
H1N1
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Molecular weight
The recombinant influenza A H1N1 Virus (A/Puerto Rico/8/34/Mount Sinai) NS1 protein comprises 240 amino acids and has a predicted molecular mass of 27.2 kDa. It migrates as an approximately 29 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Non-structural / NS1 Protein (His Tag) SDS-PAGE
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