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Product Name
NMT1 Polyclonal Antibody
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Description
Polyclonal antibody to NMT1
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
NMT1 antibody; NMT antibody; N-myristoyltransferase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NMT1 (NP_066565.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - NMT1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NMT1 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - NMT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat ovary using NMT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NMT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human colon using NMT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - NMT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using NMT1 antibody at dilution of 1:100 (40x lens).
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Background
Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"