-
Product Name
NLRC4 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to NLRC4
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
NLRC4 antibody; AIFEC antibody; CARD12 antibody; CLAN antibody; CLAN1 antibody; CLANA antibody; CLANB antibody; CLANC antibody; CLAND antibody; CLR2.1 antibody; FCAS4 antibody; IPAF antibody; NLR family CARD domain containing 4 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NLRC4 (NP_067032.3).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - NLRC4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NLRC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - NLRC4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using NLRC4 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - NLRC4 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using NLRC4 antibody . Blue: DAPI for nuclear staining.
-
Background
Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"