Human NMNAT1 (His Tag) recombinant protein

Human NMNAT1 (His Tag) recombinant protein

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Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Protects against axonal degeneration following mechanical or toxic insults.

Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1

NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE 1; 5730441G13RIK; RP11-807G9.1; NMNAT; LCA9; NICOTINAMIDE NUCLEOTIDE ADENYLYLTRANSFERASE 1; D4COLE1E; NMN ADENYLYLTRANSFERASE 1; NICOTINATE-NUCLEOTIDE ADENYLYLTRANSFERASE 1; NAD(+) PYROPHOSPHORYLASE; NAMN ADENYLYLTRANSFERASE 1; DEAMIDO-NAD(+) PYROPHOSPHORYLASE; 2610529L11RIK; NAD(+) DIPHOSPHORYLASE; PNAT1; NMNAT1; NICOTINAMIDE/NICOTINATE MONONUCLEOTIDE ADENYLYLTRANSFERASE 1; NICOTINAMIDE MONONUCLEOTIDE ADENYLYL TRANSFERASE; DEAMIDO-NAD(+) DIPHOSPHORYLASE; RP23-173D8.7; PYRIDINE NUCLEOTIDE ADENYLYLTRANSFERASE 1

Q9HAN9

NMNAT1; NMNAT

Baculovirus-Insect Cells

A DNA sequence encoding the human NMNAT1 (Q9HAN9)( Met 1-Thr279) was expressed with a C-terminal polyhistidine tag.

Human

The secreted recombinant human NMNAT1 consists of 289 amino acids and predicts a molecular mass of 33.3 KDa. The apparent molecular mass of the protein is approximately 34 KDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE