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Product Name
NEUROG3 Polyclonal Antibody
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Description
Polyclonal antibody to NEUROG3
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
NEUROG3 antibody; Atoh5 antibody; Math4B antibody; NGN-3 antibody; bHLHa7 antibody; ngn3 antibody; neurogenin-3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-83 of human NEUROG3 (NP_066279.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IF 1:20 - 1:100 -
Validations
Western blot - NEUROG3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using NEUROG3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
Acts as a transcriptional regulator. Together with NKX2-2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"