Nal-P-113 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Nal-P-113 is a synthetic antimicrobial peptide derived from P-113 (AKRHHGYKRKFH-NH2), a 12-amino-acid histidine-rich peptide originating from the salivary protein histatin 5. In Nal-P-113, the histidine residues at positions 4, 5, and 12 are replaced with β-naphthylalanine, a bulky amino acid, yielding the sequence Ac-AKR-Nal-Nal-GYKRKF-Nal-NH2. This structural modification confers resistance to high-salt conditions and degradation in biological fluids such as PBS, saliva, and serum, where the parent peptide P-113 is highly degradable.

Nal-P-113 exhibits bactericidal activity by permeabilizing and/or forming pores within cytoplasmic membranes, and its antimicrobial and anti-biofilm effects have been confirmed in in vitro studies. The peptide also displays concentration-dependent modulation of immune-inflammatory mediators, including IL-1β and TNF-α. Its ability to retain activity in physiological environments distinguishes it from many antimicrobial peptides that are effective in vitro but susceptible to degradation in vivo.

• Wang HY, Lin L, Fu W, Yu HY, Yu N, Tan LS, Cheng JW, Pan YP. Preventive effects of the novel antimicrobial peptide Nal-P-113 in a rat Periodontitis model by limiting the growth of Porphyromonas gingivalis and modulating IL-1β and TNF-α production. BMC Complement Altern Med. 2017 Aug 29;17(1):426. doi: 10.1186/s12906-017-1931-9. PMID: 28851350; PMCID: PMC5576277.
• Wang HY, Lin L, Tan LS, Yu HY, Cheng JW, Pan YP. Molecular pathways underlying inhibitory effect of antimicrobial peptide Nal-P-113 on bacteria biofilms formation of Porphyromonas gingivalis W83 by DNA microarray. BMC Microbiol. 2017 Feb 17;17(1):37.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.