NA-CATH peptide

NA-CATH peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KRFKKFFKKLKNSVKKRAKKFFKKPKVIGVTFPF

Lys-Arg-Phe-Lys-Lys-Phe-Phe-Lys-Lys-Leu-Lys-Asn-Ser-Val-Lys-Lys-Arg-Ala-Lys-Lys-Phe-Phe-Lys-Lys-Pro-Lys-Val-Ile-Gly-Val-Thr-Phe-Pro-Phe

34

95.7%

Synthetic

Powder

Discovered in 2008 (see the ref), this peptide is rich in lysines (38%). NA-CATH is predominantly alpha-helical in SDS and TFE (Dean S et al., 2011a). Active against Gram- and Gram+ bacteria (A. actinomycetemcomitans, E. coli, F. novicida, P. aeruginosa, and S. aureus) (Amer L et al., 2010;de Latour F et al., 2010; Dean S et al., 2011a;Dean S et al., 2011b) at concentrations < 1 µM). NA-CATH has low toxicity against host cells (de Latour F et al., 2010). It also has anti-biofilm activity against S. aureus (Dean S et al., 2011a). These studies also showed that small 11-aa peptide variants based on a repeated pattern within NA-CATH (KR(F/A)KKFFKK(L/P)K) have similar antimicrobial and anti-biofilm activity to the parent peptide, but not when proline is present (de Latour F et al., 2010; van Hoek M, 2014). Updated and written by Monique L. van Hoek 5Jan2015; 2/2015. NA-CATH has Antimicrobial activity. The source of NA-CATH is Naja atra.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Zhao H, Gan TX, Liu XD, et al. Identification and characterization of novel reptile cathelicidins from elapid snakes. Peptides. 2008;29(10):1685-91.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.