Mouse ASAH2 (His Tag) recombinant protein

Mouse ASAH2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

N-acylsphingosine amidohydrolase 2

BCDASE; N-CDASE; AI585898; NCDASE; HNAC1; LCDASE; ASAH2; MGC129777

Q9JHE3

Asah2; mCG_13039

Human Cells

A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse ASAH2 consists of 739 amino acids and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.
The specific activity is > 3,000 pmoles/min/μg.