Myoinhibitory neuropeptide, Pea-MIP

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Myoinhibitory neuropeptides, specifically those belonging to the MIP/AST family, are a group of pleiotropic neuropeptides in insects. They are characterized by their myoinhibitory properties on visceral muscles, such as those of the ovary and hindgut. These peptides are part of the larger allatostatin neuropeptide families, which also include FGL/AST and PISCF/AST types, and they function as signaling molecules within the insect nervous system. The MIP/AST peptides are known to regulate physiological processes including muscle contractility and enzyme activity in the gut.

In studies on tenebrionid beetles like Tenebrio molitor and Zophobas atratus, MIP/AST peptides, such as Grybi-MIP1, have been observed to influence the contractile activity of visceral organs. For instance, Grybi-MIP1 exhibits myostimulatory effects on the oviduct and hindgut muscles in these beetles, with dose-dependent and tissue-specific responses. This peptide increases the frequency of contractions in these tissues, particularly at low physiological concentrations, highlighting its role in modulating reproductive and digestive functions in beetles.

• Lubawy J, Marciniak P, Kuczer M, Rosiński G. Myotropic activity of allatostatins in tenebrionid beetles. Neuropeptides. 2018 Aug;70:26-36. doi: 10.1016/j.npep.2018.05.003. Epub 2018 May 9.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.