Mouse TROP-2/TACSTD2 (His Tag) recombinant protein

Mouse TROP-2/TACSTD2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate.

LY97; GP50; MGC141613; EGP1; TACSTD2; GA733-1; GA7331; M1S1; TROP2; MGC141612; EGP-1; C80403

Human Cells

A DNA sequence encoding the extracellular domain of mouse TACSTD2 (Q8BGV3?) (Met 1-Gln 270) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse TACSTD2 comprises 257 amino acids and has a calculated molecular mass of 29.4 kDa. As a result of glycosylation, the apparent molecular mass of rmTACSTD2 is approximately 45 kDa in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 10E4 cells/well are added to TACSTD2 coated plates (40 μg/mL with 100 μL/well), > 50% cells will adhere for 1 hour incubation at 37℃.