Mouse TNFR1/CD120a/TNFRSF1A (Fc Tag) recombinant protein

Mouse TNFR1/CD120a/TNFRSF1A (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

P55; TNF-R-I; FPF; MGC19588; CD120A; TNFAR; P55-R; TNF-R55; TNFR60; TBP1; TNF-R; TNFR1; TNFRSF1A; TNFR55; P60

Human Cells

A DNA sequence encoding the mouse TNFRSF1A (NP_ 035739.2) extracellular domain (Met 1-Ala 212) was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse TNFRSF1A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 424 amino acids and has a predicted molecular mass of 47.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm TNFRSF1A/Fc monomer is approximately 55 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

Immobilized mouse TNFa (80-235) (Cat:500439 ) at 10 μg/ml (100 μl/well) can bind mouse TNFRSF1A-Fc, The EC₅₀ of mouse TNFRSF1A-Fc is 0.03-0.07 μg/ml.