Mouse NEK3 (His & GST Tag) recombinant protein

Mouse NEK3 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for NEK3 gene. NEK3 may play a role in mitotic regulation.

NIMA-RELATED PROTEIN KINASE 3; SERINE/THREONINE-PROTEIN KINASE NEK3; NEVER IN MITOSIS A-RELATED KINASE 3; PHOSPHORYLASE B KINASE KINASE; HSPK36; HSPK 36; GLYCOGEN SYNTHASE A KINASE; NIMA-RELATED EXPRESSED KINASE 3; HYDROXYALKYL-PROTEIN KINASE

Baculovirus-Insect Cells

A DNA sequence encoding the mouse NEK3 (ABK42288.1) (Met 1-Ala 509) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Mouse

The secreted recombinant mouse NEK3/GST chimera consists of 746 amino acids and has a calculated molecular mass of 84.8 kDa. The recombinant protein migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.

> 82 % as determined by SDS-PAGE

The specific activity was determined to be 8 nmol/min/mg using MBP as substrate.