Mouse LIF recombinant protein

Mouse LIF recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin.

RP23-329P17.1; HILDA; MLPLI; CDF; OTTMUSP00000005253; D FACTOR; DIA

P09056

Human Cells

A DNA sequence encoding the mouse LIF (NP_032527.1) (Met1-Phe203) was expressed and purified.

Mouse

The recombinant mouse LIF consists of 180 amino acids and predicts a molecular mass of 19.9 kDa. It migrates as approximately 34.1 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.
The ED₅₀ for this effect is typically 0.1-0.5 ng/mL.