Mouse CD59a / Protectin (His Tag) recombinant protein

Mouse CD59a / Protectin (His Tag) recombinant protein

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Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins.It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.

B-cell differentiation antigen CD72

CD59; PROTECTIN; AA987121; RP24-297H17.1

P21855

Cd72; Ly-32; Ly32; Lyb-2

Human Cells

A DNA sequence encoding the mouse CD59a (NP_031680.2) (Met 1-Lys 95), without the pro peptide, was fused with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse CD59a comprises 83 amino acids and has a calculated molecular mass of 9.7 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE