Mouse CD297 (His Tag) recombinant protein

Mouse CD297 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.

NAD(P)(+)--ARGININE ADP-RIBOSYLTRANSFERASE 4; MONO(ADP-RIBOSYL)TRANSFERASE 4; DOK1; DO; CD297; ARTC4; MONO-ADP-RIBOSYLTRANSFERASE 4; ECTO-ADP-RIBOSYLTRANSFERASE 4; MONO(ADP-RIBOSYL)TRANSFERASE; 4432404K01RIK; DOMBROCK BLOOD GROUP CARRIER MOLECULE; ADP-RIBOSYLTRANSFERASE 4 (DO BLOOD GROUP)

Human Cells

A DNA sequence encoding the mature form of mouse ART4 (EDL10575.1) (Met1-Lys 263) was expressed, with a C-terminal polyhistidine tag.

Mouse

The recombinant mouse ART4 consists of 251 amino acids and predicts a molecular mass of 28.2 KDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmART4 is approximately 40-45 KDa as a result of glycosylation.

> 92 % as determined by SDS-PAGE