Mouse BTLA (Fc Tag) recombinant protein

Mouse BTLA (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

BTLA is a inhibitory molecule which belongs to the Ig superfamily. It down-modulates immune responses. As such, reagents that regulate the binding of BTLA to its ligand or alter BTLA signaling have significant therapeutic promise. BTLA is crucial to understand the mechanism(s) of action of these antibodies before attempting clinical applications. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).

A630002H24; CD272; B- AND T-LYMPHOCYTE ATTENUATOR; BTLA1; B- AND T-LYMPHOCYTE-ASSOCIATED PROTEIN; B AND T LYMPHOCYTE ATTENUATOR

Human Cells

A DNA sequence encoding the mouse BTLA (Met1-Gly176) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse BTLA /Fc is a disulfide-linked homodimer. The reduced monomer comprises 388 amino acids and has a predicted molecular mass of 437 kDa. The apparent molecular mass of the protein is approximately 57-62 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Immobilized mouse BTLA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated mouse HVEM-Fch (Cat:503760), The EC₅₀ of biotinylated mouse HVEM-Fch (Cat:503760) is 15.7-36.7 ng/ml.