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Product Name
Mouse Alpha 1 Antitrypsin / Serpin A1a recombinant protein
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Protein short names
ALPHA-1-ANTIPROTEINASE; CLADE A; AAT; SPI1-1; SERPIN A1A; AAT-2; SERINE PROTEASE INHIBITOR A1A; SERPINA1A; ALPHA-1-ANTITRYPSIN 2; AAT2; SERINE (OR CYSTEINE) PEPTIDASE INHIBITOR; SERINE PROTEASE INHIBITOR 1-1; SPI1-3; SERINE (OR CYSTEINE) PROTEINASE INHIBITOR; ALPHA-1 PROTEASE INHIBITOR 1; DOM1; PI1; ALPHA-1-ANTITRYPSIN 1-1; MEMBER 1A
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the mouse Serpina1a (XP_003945711.1) (Met1-Lys413) was expressed with five amino acids (DDDDK) at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse Serpina1a comprises 395 amino acids and has a predicted molecular mass of 44.1 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate,Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys (DNP)-NH2.
The IC50 value is < 15 nM. -
Validations
Mouse Alpha 1 Antitrypsin / SerpinA1a Protein SDS-PAGE
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