mineralization nucleation (HAP) peptide

mineralization nucleation (HAP) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DDDEEKFLRRIGFG-OH

H-Asp-Asp-Asp-Glu-Glu-Lys-Phe-Leu-Arg-Arg-Ile-Gly-Phe-Gly-OH

14

95.51%

C₇₄H₁₁₃N₂₁O₂₅

1696.8

Synthetic

Powder

DDDEEKFLRRIGFG polypeptide is known for its role in nucleation of mineralization, which is crucial for processes like bone regeneration. It can be incorporated into elastin-like recombinamer (ELR) molecules, enhancing their bioactivity. The resulting ELR membranes with DDDEEKFLRRIGFG have shown promising in vitro properties, such as the ability to promote osteogenic differentiation of cells. They also exhibit good mechanical properties and biocompatibility due to the characteristics of the ELR structure. Overall, DDDEEKFLRRIGFG polypeptide offers a valuable option for creating bioactive materials that could have significant implications in the field of tissue engineering and regenerative medicine.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Tejeda-Montes E, Klymov A, Nejadnik MR, Alonso M, Rodriguez-Cabello JC, Walboomers XF, Mata A. Mineralization and bone regeneration using a bioactive elastin-like recombinamer membrane. Biomaterials. 2014 Sep;35(29):8339-47. doi: 10.1016/j.biomaterials.2014.05.095. Epub 2014 Jul 2. PMID: 24996755.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.