MERS-CoV CoV Spike glycoprotein (aa 383-502, Fc Tag)

MERS-CoV CoV Spike glycoprotein (aa 383-502, Fc Tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. 

S protein

K0BRG7

S

Baculovirus-Insect Cells

A DNA sequence encoding the spike protein fragment (Human betacoronavirus 2c EMC/2012)(AFS88936.1)(Cys383-Lys502) was fused with the Fc region of rabbit IgG at the C-terminus.

MERS-CoV

The recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012) comprises 344 amino acids and has a predicted molecular mass of 38.5 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
1. Immobilized Spike (HCoV-EMC/2012) (Cat: 503810) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc (Cat: 501568). The EC₅₀ of biotinylated human ACE2-Fc (Cat: 501568) is 0.04-0.06 μg/ml.
2. Immobilized Spike (HCoV-EMC/2012) (Cat: 503810) at 10 μg/ml (100 μl/well) can bind biotinylated DPP4 (Cat: 502818). The EC₅₀ of of biotinylated DPP4 (Cat: 502818) is 0.10-0.16 μg/ml.
3. Immobilized Spike (HCoV-EMC/2012) (Cat: 503810) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 504194). The EC₅₀ of biotinylated Fc-DPP4 (Cat: 504194) is 0.15-0.23 μg/ml.