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Product Name
MEPE antibody
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Description
MEPE Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, A2780 cells, HeLa cells. Observed molecular weight by Western-blot: 43kd,98kd
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Tested applications
ELISA, WB
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Species reactivity
human; other species not tested.
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Alternative names
MEPE antibody; Osteoblast/osteOCyte factor 45 antibody; OF45 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_020203). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112658(MEPE antibody) at dilution of 1:600
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Background
Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases. (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).
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