Melectin peptide

Melectin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

GFLSILKKVLPKVMAHMK

Gly-Phe-Leu-Ser-Ile-Leu-Lys-Lys-Val-Leu-Pro-Lys-Val-Met-Ala-His-Met-Lys

18

96.3%

Synthetic

Powder

Using synthetic peptides, Pro11 was found to be important for peptide selectivity. Changes of Pro11 to Ala, Lys, and Gly or delection of Pro11 had little effect on antibacterial activities but increased the hemolytic activities. The antibacterial region is located at the N-terminus of the peptide (a peptide containing residues 1-10 is active). The peptide also exhibits mast cell degranulating activity. Also,the peptide was recently found to be HIV inhibitory (EC50=4.3 uM) (Wang G et al. 2010 Antimicrob. Agents Chemother. 54: 1343-1346). Melectin has Antibacterial activity. The source of Melectin is the Cleptoparasitic bee Melecta albifrons.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Cerovský V, Hovorka O, Cvacka J, et al. Melectin: a novel antimicrobial peptide from the venom of the cleptoparasitic bee Melecta albifrons. Chembiochem. 2008;9(17):2815-21.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.