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Product Name
MEF2C antibody
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Description
MEF2C Rabbit Polyclonal antibody. Positive WB detected in SH-SY5Y cells, mouse colon tissue. Positive IP detected in SH-SY5Y cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 52 kDa; 63kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MEF2C antibody; myOCyte enhancer factor 2C antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MEF2C recombinant protein (Accession Number: XM_047417182). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:112632(MEF2C antibody) at dilution of 1:500
IP Result of anti-MEF2C (IP:Catalog No:112632, 3ug; Detection:Catalog No:112632 1:500) with SH-SY5Y cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112632(MEF2C antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:112632(MEF2C Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. Read more about this antibody on the blog:http://blog.ptglab.com/index.php/mef2c-antibody-features-in-nature-biotechnology/
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References
- Martinez-Fernandez A, Nelson TJ, Yamada S. iPS programmed without c-MYC yield proficient cardiogenesis for functional heart chimerism. Circulation research. 105(7):648-56. 2009.
- Martinez-Fernandez A, Nelson TJ, Ikeda Y, Terzic A. c-MYC independent nuclear reprogramming favors cardiogenic potential of induced pluripotent stem cells. Journal of cardiovascular translational research. 3(1):13-23. 2010.
- Waclaw RR, Ehrman LA, Pierani A, Campbell K. Developmental origin of the neuronal subtypes that comprise the amygdalar fear circuit in the mouse. The Journal of neuroscience : the official journal of the Society for Neuroscience. 30(20):6944-53. 2010.
- Cocas LA, Georgala PA, Mangin JM. Pax6 is required at the telencephalic pallial-subpallial boundary for the generation of neuronal diversity in the postnatal limbic system. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(14):5313-24. 2011.
- Teissier A, Waclaw RR, Griveau A, Campbell K, Pierani A. Tangentially migrating transient glutamatergic neurons control neurogenesis and maintenance of cerebral cortical progenitor pools. Cerebral cortex (New York, N.Y. : 1991). 22(2):403-16. 2012.
- Shi JH, Zheng B, Chen S, Ma GY, Wen JK. Retinoic acid receptor α mediates all-trans-retinoic acid-induced Klf4 gene expression by regulating Klf4 promoter activity in vascular smooth muscle cells. The Journal of biological chemistry. 287(14):10799-811. 2012.
- Tang K, Rubenstein JL, Tsai SY, Tsai MJ. COUP-TFII controls amygdala patterning by regulating neuropilin expression. Development (Cambridge, England). 139(9):1630-9. 2012.
- Drukker M, Tang C, Ardehali R. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nature biotechnology. 30(6):531-42. 2012.
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