Medusin-PH peptide

Medusin-PH peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-LLGMIPVAISAISALSKL-OH

H-Leu-Leu-Gly-Met-Ile-Pro-Val-Ala-Ile-Ser-Ala-Ile-Ser-Ala-Leu-Ser-Lys-Leu-OH

18

95.42%

C₈₃H₁₄₉N₁₉O₂₂S

1797.24

Synthetic

Powder

Medusin-PH is a novel antimicrobial peptide derived from the skin secretions of the South American orange-legged leaf frog (Phyllomedusa hypochondrialis).
Medusin-PH exhibits growth inhibitory activity against Gram-positive bacterium Staphylococcus aureus and yeast Candida albicans.
Medusin-PH has a minimum inhibitory concentration of 32 mg/L against S. aureus and 128 mg/L against C. albicans.
Compared to other peptides like phylloseptin-1, Medusin-PH shows differences in bioactivity, being inactive against Gram-negative bacteria and having slightly lower MIC for Gram-positive bacteria.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Xi X, Li R, Jiang Y, et al. Medusins: a new class of antimicrobial peptides from the skin secretions of phyllomedusine frogs. Biochimie. 2013;95(6):1288-96.
• Proaño-Bolaños C, Blasco-Zúñiga A, Almeida JR, Wang L, Llumiquinga MA, Rivera M, Zhou M, Chen T, Shaw C. Unravelling the Skin Secretion Peptides of the Gliding Leaf Frog, Agalychnis spurrelli (Hylidae). Biomolecules. 2019 Oct 30;9(11):667.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.