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Product Name
MED8 antibody
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Description
MED8 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, mouse liver tissue, SGC-7901 cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ARC32 antibody; MED8 antibody; mediator complex subunit 8 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MED8 recombinant protein (Accession Number: BC010543). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:112594(MED8 antibody) at dilution of 1:1000
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Background
MED8, a component of the Mediator complex, is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. It plays a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. MED8 has three isoforms with MW 19 kDa, 29 kDa and 33 kDa. And the ubiquitination form will be 38-43 kDa.
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