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Product Name
Recombinant MDM2 protein (His tag)
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Description
Recombinant Human MDM2 (1-111) Fragment: A high-purity recombinant protein encompassing the SWIB/MDM2 domain (residues 26-109) of E3 ubiquitin-protein ligase Mdm2. This fragment serves as an essential tool for studying the specific molecular functions of this critical regulatory domain involved in p53 pathway modulation and protein-protein interactions.
MDM2 (E3 ubiquitin-protein ligase Mdm2) is a crucial regulatory protein primarily known for its role as a negative regulator of the tumor suppressor p53. As a RING-type E3 ubiquitin transferase, MDM2 mediates the ubiquitination of p53, leading to its proteasomal degradation, thereby inhibiting p53-mediated cell cycle arrest and apoptosis. Beyond its canonical p53 regulation, MDM2 exhibits diverse functions including self-ubiquitination, ubiquitination of ARRB1, promotion of nuclear export of p53, and proteasome-dependent degradation of retinoblastoma RB1 protein. The protein localizes predominantly to the nucleoplasm but also shows cytoplasmic and nuc
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Protein name
E3 ubiquitin-protein ligase Mdm2
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Uniprot ID
Q00987
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Gene Name
MDM2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-111 aa (Q00987) were fused with N-terminal 6His tag.
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Sequence
MNHKVHHHHH HGGSMCNTNM SVPTDGAVTT SQIPASEQET LVRPKPLLLK LLKSVGAQKD TYTMKEVLFY LGQYIMTKRL YDEKQQHIVY CSNDLLGDLF GVPSFSVKEH RKIYTMIYRN LVVVN
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 14.36 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 14 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant MDM2 protein (His tag) SDS-PAGE
M: Marker; Lane 1: MDM2; Lane 2: BSA
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"