-
Product Name
MCT1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to MCT1
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
SLC16A1 antibody; HHF7 antibody; MCT antibody; MCT1 antibody; MCT1D antibody; monocarboxylate transporter 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide of human MCT1
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - MCT1 Polyclonal Antibody
Western blot analysis of extracts of mouse seminoma, using MCT1 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - MCT1 Polyclonal Antibody
Western blot analysis of extracts of various cells, using MCT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - MCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using MCT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human tonsil using MCT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using MCT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat pancreas using MCT1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - MCT1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver using MCT1 antibody at dilution of 1:100 (40x lens).
-
Background
Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"