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Product Name
MCRS1 antibody
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Description
MCRS1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human brain tissue, human liver tissue, human spleen tissue, Jurkat cells, mouse spleen tissue, mouse uterus tissue, rat spleen tissue. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
58 kDa microspherule protein antibody; ICP22BP antibody; INO80 complex subunit J antibody; INO80Q antibody; MCRS1 antibody; MCRS2 antibody; microspherule protein 1 antibody; MSP58 antibody; P78 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MCRS1 recombinant protein (Accession Number: NM_006337). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112561(MCRS1 antibody) at dilution of 1:500
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Background
MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.
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References
- Chen L, Cai Y, Jin J. Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. The Journal of biological chemistry. 286(13):11283-9. 2011.
- Chelmicki T, Dündar F, Turley MJ. MOF-associated complexes ensure stem cell identity and Xist repression. eLife. 3:e02024. 2014.
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