Mastoparan-VT6 peptide

Mastoparan-VT6 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

INLKAIAALVKKLL

Ile-Asn-Leu-Lys-Ala-Ile-Ala-Ala-Leu-Val-Lys-Lys-Leu-Leu

14

97%

Synthetic

Powder

Active against Gram-positive S. aureus ATCC25923 (MIC 20 uM), E. faecalis ATCC29212, S. aureus (IS 10#) (MIC 80 uM), S. aureus (IS 37#) (MIC 20 uM), Gram-negative E. cloacae ATCC13047 (MIC 80 uM), K. pneumonia ATCC700603 (MIC 40 uM), P. aeruginosa ATCC27853 (MIC 40 uM), B. dysenteriae (MIC 20 uM), E. coli (IS 37#) (MIC 40 uM), E. coli (IS 218#) (MIC 80 uM), P. aeruginosa (IS 350#) (MIC 40 uM), fungi C. albicans ATCC2002 (MIC 80 uM), C. albicans ATCC90028 (MIC 40 uM), and C. parapsilosis ATCC22019 (MIC 40 uM). Mastoparan-VT6 was found in venom gland, the social wasp, Vespa tropica.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yang X, Wang Y, Lee WH, Zhang Y. Antimicrobial peptides from the venom gland of the social wasp Vespa tropica. Toxicon. 2013;74:151-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.