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Product Name
Marmoset IL23 (IL23A & IL12B Heterodimer) recombinant protein
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the marmoset IL23A (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the marmoset IL12B (Met 1-Asn328) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the marmoset IL23A & the marmoset IL12B heterodimer was purified.
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Protein Species
Marmoset
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Molecular weight
The recombinant marmoset IL23A & the marmoset IL12B comprises 498(181+317) amino acids and has a calculated molecular mass of 56.4 (20.1+36.3) KDa. The apparent molecular mass of the marmoset IL23A & the marmoset IL12B heterodimer is approximately 22 & 45 KDa respectively in SDS-PAGE.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized marmoset IL23A-His+marmoset IL12B-His (Cat:CT037-C08H) at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His (Cat:501974). The EC50 of biotinylated human IL12RB1-His (Cat:501974) is 55-129 μg/ml. -
Validations
Marmoset IL-23 (IL23A & IL12B Heterodimer) Protein SDS-PAGE
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