MANS peptide
Not For Human Use, Lab Use Only.
Cat.#: 319489
Special Price 238.3 USD
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Product Name
MANS peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
Myrisitic Acid-GAQFSKTAAKGEAAAERPGEAAVA-OH
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Sequence
Myrisitic Acid-Gly-Ala-Gln-Phe-Ser-Lys-Thr-Ala-Ala-Lys-Gly-Glu-Ala-Ala-Ala-Glu-Arg-Pro-Gly-Glu-Ala-Ala-Val-Ala-OH
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Length (aa)
24
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Peptide Purity (HPLC)
95.06%
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Molecular Formula
C111H184N30O35
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Molecular Weight
2498.81
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Source
Synthetic
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Form
Powder
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Description
Myristoylated alanine-rich C kinase substrate (MARCKS) is a ubiquitously expressed protein kinase C substrate that has emerged as a potential therapeutic target for the amelioration of mucin secretion and inflammation in patients with chronic obstructive pulmonary disease. MANS (myristoylated N-terminal sequence) is the well-characterized human MARCKS inhibitory peptide. Pretreatment of canine neutrophils with MANS peptide significantly reduced both mRNA and protein expression in a broad range of LPS-induced cytokines, including IL-8, a chemokine (C-X-C motif) ligand–1 orthologue, and TNF-α, in comparison with untreated cells or those treated with a control peptide.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Li J, D'Annibale-Tolhurst MA, Adler KB, Fang S, Yin Q, Birkenheuer AJ, Levy MG, Jones SL, Sung EJ, Hawkins EC, Yoder JA, Nordone SK. A myristoylated alanine-rich C kinase substrate-related peptide suppresses cytokine mRNA and protein expression in LPS-activated canine neutrophils. Am J Respir Cell Mol Biol. 2013 Mar;48(3):314-21. doi: 10.1165/rcmb.2012-0278OC. Epub 2012 Dec 6. PMID: 23221047; PMCID: PMC3604091.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
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• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"