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Product Name
MAGEA6 Polyclonal Antibody
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Description
Polyclonal antibody to MAGEA6
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
MAGEA6 antibody; CT1.6 antibody; MAGE-3b antibody; MAGE3B antibody; MAGE6 antibody; MAGE family member A6 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 75-314 of human MAGEA6 (NP_005354.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - MAGEA6 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using MAGEA6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - MAGEA6 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using MAGEA6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"