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Product Name
MACF1 antibody
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Description
MACF1 Rabbit Polyclonal antibody. Positive WB detected in Transfected HEK-293 cells. Positive IHC detected in human prostate cancer tissue, human heart tissue, human skeletal muscle tissue. Positive IF detected in RPE cells, NIH/3T3 cells.
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
620 kDa actin binding protein antibody; ABP620 antibody; ACF7 antibody; FLJ45612 antibody; FLJ46776 antibody; KIAA0465 antibody; KIAA1251 antibody; MACF antibody; MACF1 antibody; Macrophin 1 antibody; OFC4 antibody; Trabeculin alpha antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MACF1 recombinant protein (Accession Number: BC007330). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: N/A
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Validations
IF results (golgi stain) of MACF1 antibody (1:100) with RPE cells (fixed with 4% formaldehyde) by O’Neil Wiggan, Colorado State Univ.
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112365(MACF1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:112365(MACF1 antibody) at dilution of 1:100 (under 40x lens)
Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112365(MACF1 Antibody) at dilution of 1:1000
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Background
MACF1, also called ACF7 (actin crosslinking family 7), is a member of the spectraplakin family and is a ubiquitously expressed 614 kDa protein. This protein facilitates actin-microtubule interactions at the cell periphery and couples the microtubule network to cellular junctions. It displays a conserved role in membrane protrusion formation during evolution. The two major MACF1 isoforms characterized to date, MACF1a and MACF1b, result from alternative splicing. Both proteins contain N-terminal actin-binding domains (ABD), followed by plakin domains, extended spectrin repeat domains, EF-hand motifs, and C-terminal microtubule-binding domains (MTBDs). MACF1 is expressed in numerous tissue, including the skin, brain, skeletal muscle, and heart.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"