LRELHLNNN peptide

LRELHLNNN peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-LRELHLNNN-OH

H-Leu-Arg-Glu-Leu-His-Leu-Asn-Asn-Asn-OH

9

95.38%

C₄₇H₇₉N₁₇O₁₅

1122.23

Synthetic

Powder

Peptide LRELHLNNN is a small chain of amino acids that has been identified as having the ability to selectively bind to collagen type I, which is an important protein in fibrosis. Fibrosis refers to the pathological formation of excess connective tissue and can occur in many diseases including liver and pancreas disease. The affinity (or strength) of binding between peptide LRELHLNNN and collagen type I was measured at 170 nM. Researchers have modified this peptide by attaching it to a molecule called DOTA/NOTA via a PEG2-linker. This modification allows for radiolabeling with Gallium-68 or aluminium fluoride-18, making it useful as a PET tracer for detecting fibrotic lesions non-invasively.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Maria, Rosestedt., Irina, Velikyan., Ulrika, Rosenström., Sergio, Estrada., Ola, Åberg., Jan, Weis., Christer, Westerlund., Sofie, Ingvast., Olle, Korsgren., Patrik, Nordeman., Olof, Eriksson. (2021). Radiolabelling and positron emission tomography imaging of a high-affinity peptide binder to collagen type 1.. Nuclear Medicine and Biology, 93:54-62. doi: 10.1016/J.NUCMEDBIO.2020.11.006

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.