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Product Name
LRCH1 antibody
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Description
LRCH1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IF detected in MCF-7 cells. Positive IHC detected in human cervical cancer tissue. Observed molecular weight by Western-blot: 85-95 kDa
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Tested applications
ELISA, WB, IP, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
CHDC1 antibody; KIAA1016 antibody; LRCH1 antibody; Neuronal protein 81 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LRCH1 recombinant protein (Accession Number: NM_001164213). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112314(LRCH1 Antibody) at dilution of 1:1000
IP Result of anti-LRCH1 (IP:Catalog No:112314, 4ug; Detection:Catalog No:112314 1:300) with HeLa cells lysate 1600ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:112314(LRCH1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:112314(LRCH1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of MCF-7 cells using Catalog No:112314(LRCH1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
LRCH1, also named as CHDC1 or Neuronal protein 81, is a 728 amino acid protein, which contains one CH domain and nine LRR repeats. The molecular weight of LRCH1 is 81 kDa and it contains phosphorylation modified residues. we detected a 85-95 kDa protein by western blot. The function of LRCH1 is still non-known.
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