-
Product Name
Mouse LDL Receptor (His Tag) recombinant protein
- Documents
-
Description
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.
-
Protein name
Low-density lipoprotein receptor
-
Protein short names
HLB301; LDLR; FH; FHC; LDLCQ2
-
Uniprot ID
P35951
-
Gene Name
Ldlr
-
Source/Expression Host
Human Cells
-
Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse LDLR (NP_034830.2) precursor (Met 1-Arg 790) with substitution of Val 23 and Gly 27 by Ala 23 and Cys 27 respectively was expressed with a C-terminal polyhistidine tag.
-
Protein Species
Mouse
-
Molecular weight
The secreted recombinant mouse LDLR comprises 780 amino acids with a predicted molecular mass of 85.7 kDa. As a result of glycosylation, it migarates as approximately 120-130 kDa protein in SDS-PAGE under reducing conditions.
-
Purity
> 90 % as determined by SDS-PAGE
-
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant mouse LDLR. The EC50 of biotinylated mouse LDLR is 0.173 μg/ml.
-
Validations
Mouse LDLR / LDL Receptor Protein (His Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"