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Product Name
E. coli LON PROTEASE (His Tag) recombinant protein
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Description
ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, UmuD and antitoxins CcdA and HipB. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system.
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Protein name
Lon protease
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Uniprot ID
P0A9M0
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Gene Name
lon; capR; deg; lopA; muc; b0439; JW0429
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding E.coil Lon (P0A9M0) (Met1-Lys784) was expressed with a polyhistidine tag at the C-terminus.
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Protein Species
E. coli
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Molecular weight
The recombinant E.coil lon consists of 790 amino acids and predicts a molecular mass of 88.3 KDa. It migrates as an approximately 88 KDa band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Validations
E.coli Lon protease Protein (His Tag) SDS-PAGE
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