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Product Name
LKB1 antibody
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Description
LKB1 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, Jurkat cells. Positive IHC detected in human lymphoma tissue, human liver tissue, human lung cancer tissue, human skin tissue, human testis tissue. Observed molecular weight by Western-blot: 54 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
LKB1 antibody; PJS antibody; serine/threonine kinase 11 antibody; STK11 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LKB1 recombinant protein (Accession Number: NM_000455). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:112249(STK11 antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:112249(STK11 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-STK11 (IP:Catalog No:112249, 4ug; Detection:Catalog No:112249 1:500) with HEK-293 cells lysate 2000ug.
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Background
STK11(serine/threonine-protein kinase 11) is also named as LKB1,PJS,and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response.The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS).Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.
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References
- Zhao F, Huang W, Zhang Z. Triptolide induces protective autophagy through activation of the CaMKKβ-AMPK signaling pathway in prostate cancer cells. Oncotarget. 2015.
- Nakada Y, Stewart TG, Peña CG. The LKB1 tumor suppressor as a biomarker in mouse and human tissues. PloS one. 8(9):e73449. 2013.
- Yang JY, Jiang SH, Liu DJ. Decreased LKB1 predicts poor prognosis in Pancreatic Ductal Adenocarcinoma. Scientific reports. 5:10575. 2015.
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