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Product Name
LIPA antibody
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Description
LIPA Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in Y79 cells, HEK-293 cells, HeLa cells, HepG2 cells, HT-1080 cells. Observed molecular weight by Western-blot: 45kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CESD antibody; Cholesteryl esterase antibody; LAL antibody; LIPA antibody; Lipase A antibody; Sterol esterase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of LIPA recombinant protein (Accession Number: NM_000235). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:112241(LIPA antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:112241(LIPA antibody) at dilution of 1:100 (under 10x lens)
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Background
Human acid lipase/cholesteryl esterase (LIPA) is a 46-kDa glycoprotein required for the lysosomal hydrolysis of cholesteryl esters and triglycerides that cells acquire through the receptor-mediated endocytosis of low-density lipoproteins(PMID:8432549). Defects in LIPA are the cause of Wolman disease (WOD)(PMID:8146180) and cholesteryl ester storage disease (CESD)(PMID:9633819). It has 2 isoforms produced by alternative splicing with the molecular weight of 45 kDa and 39 kDa. The full length protein has a signal peptide and several glycosylation sites.
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