Human Lefty2 (Fc Tag) recombinant protein
left-right determination factor 2 (Lefty2), also known as Left-right determination factor A (Lefty A), endometrial bleeding associated factor (left-right determination, factor A transforming growth factor beta superfamily), and Transforming growth factor beta-4, is a member of the TGF-beta family of proteins. This protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. Lefty2/Lefty A may also play a role in endometrial bleeding. Mutations in Lefty2/Lefty A have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. Depletion of both Lefty1 and Lefty2 causes unchecked Nodal signaling, expansion of mesendoderm, and loss of ectoderm. The expansion of mesendoderm correlates with an extended period of rapid cellular internalization and a failure of deep-cell epiboly. The gastrulation defects of embryos depleted of Lefty1 and Lefty2 result from the deregulation of Squint signaling.
Left-right determination factor 2, isoform CRA_a
Protein short names
MGC98569; 6030463A22RIK; EBAF; LEFTYA; MGC46222; TGFB4; LEFTA; LEFTB; LEFTY2; AV214969
A DNA sequence encoding the mature form of human LeftyA (NP_003231.2) (Phe 78-Pro 366) was fused with the Fc region of human IgG1 at the N-terminus.
The recombinant human LeftyA/Fc is a disulfide-linked homodimer The reduced monomer consists of 526 amino acids and has a predicted molecular mass of 58.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LeftyA/Fc monomer is approximately 70 kDa due to glycosylation.
> 90 % as determined by SDS-PAGE
Measured by its ability to inhibit recombinant human BMP4 induced alkaline phosphatase production by MC3T3-E1 cells.
The ED50 for this effect is typically 4-16 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
Human Lefty2 / Lefty A Protein (Fc Tag) SDS-PAGE
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