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Product Name
LECT1 Polyclonal Antibody
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Description
Polyclonal antibody to LECT1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse
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Alternative names
CNMD antibody; BRICD3 antibody; CHM-I antibody; CHM1 antibody; LECT1 antibody; MYETS1 antibody; chondromodulin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 75-334 of human LECT1 (NP_008946.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:100 -
Validations
Western blot - LECT1 Polyclonal Antibody
Western blot analysis of extracts of mouse liver, using LECT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - LECT1 Polyclonal Antibody
Immunofluorescence analysis of A549 cells using LECT1 antibody . Blue: DAPI for nuclear staining.
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Background
Bifunctional growth regulator that stimulates the growth of cultured chondrocytes in the presence of basic fibroblast growth factor (FGF) but inhibits the growth of cultured vascular endothelial cells. May contribute to the rapid growth of cartilage and vascular invasion prior to the replacement of cartilage by bone during endochondral bone development. Inhibits in vitro tube formation and mobilization of endothelial cells. Plays a role as antiangiogenic factor in cardiac valves to suppress neovascularization.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"