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Product Name
KRT6B antibody
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Description
KRT6B Rabbit Polyclonal antibody. Positive IP detected in mouse skin tissue. Positive WB detected in mouse skin tissue, A431 cells. Positive IHC detected in human cervix tissue, human oesophagus tissue, human tonsil tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CK 6B antibody; CK6B antibody; Cytokeratin 6B antibody; K6B antibody; keratin 6B antibody; KRT6B antibody; KRTL1 antibody; PC2 antibody; Type II keratin Kb10 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of KRT6B recombinant protein (Accession Number: NM_005555). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skin tissue were subjected to SDS PAGE followed by western blot with Catalog No:112134(KRT6B antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervix using Catalog No:112134(KRT6B antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervix using Catalog No:112134(KRT6B antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-KRT6B (IP:Catalog No:112134, 4ug; Detection:Catalog No:112134 1:800) with mouse skin tissue lysate 3200ug.
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Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. Keratin 6 is a type II keratin. It is used as marker for epidermal hyperproliferation and differentiation. Three keratin 6 isoforms were found, ketatin 6A,6B and 6C. They share more than 99% identical DNA sequence.
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References
- Cui CY, Klar J, Georgii-Heming P. Frizzled6 deficiency disrupts the differentiation process of nail development. The Journal of investigative dermatology. 133(8):1990-7. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"