KP1 (Klotho-derived peptide 1)

KP1 (Klotho-derived peptide 1)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FQGTFPDGFLWAVGSAAYQTEGGWQQHGKG-OH

H-Phe-Gln-Gly-Thr-Phe-Pro-Asp-Gly-Phe-Leu-Trp-Ala-Val-Gly-Ser-Ala-Ala-Tyr-Gln-Thr-Glu-Gly-Gly-Trp-Gln-Gln-His-Gly-Lys-Gly-OH

30

95.38%

C₁₄₉H₂₀₃N₃₉O₄₃

3228.48

Synthetic

Powder

Klotho-derived peptide 1 hydrochloride (KP1) is a 30-amino acid peptide corresponding to Phe57–Lys86 of human Klotho, representing a buried β-strand in the N-terminal domain. Chemically synthesizable and cost-effective, KP1 recapitulates Klotho's anti-fibrotic and renoprotective actions. KP1 binds TGF-β receptor 2 (TβR2), disrupting TGF-β/TβR2 interaction and inhibiting downstream Smad2/3 and MAPK pathways. In mouse models of renal fibrosis, KP1 preserves kidney function, ameliorates fibrosis, and restores endogenous Klotho expression via posttranscriptional regulation through the miR-223-3p/lncRNA-TUG1 axis. Additionally, KP1 protects against acute kidney injury by directly targeting mitochondrial ATAD3A, preventing cytochrome c release and tubular apoptosis—independent of TGF-β signaling. KP1 demonstrates efficacy across multiple renal injury models (UUO, UIRI, cisplatin, IRI).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• [1] Zhang X, Lin S, Wu T, Zhang Z, Zhou H, Hong X, Liu Y. Klotho-Derived Peptide 1 Protects against Acute Kidney Injury by Directly Targeting Mitochondrial ATAD3A. Adv Sci. 2026 Aug 25:e77214. doi: 10.1002/advs.77214. Epub ahead of print. PMID: 42640132; PMCID: PMC13505471.
• [2] Xi Y, Han G, Li X, et al. Klotho-derived peptide preserves erectile function by limiting fibrosis, oxidative stress, and apoptosis of penile smooth muscle cells in cavernous nerve injured-rats through suppression of the TGF-β1/TGF-β type II receptor signaling. Eur J Pharmacol. 2026;1022:178849. doi:10.1016/j.ejphar.2026.178849
• [3] Yuan Q, Ren Q, Li L, et al. A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-β signaling. Nat Commun. 2022;13(1):438. Published 2022 Jan 21. doi:10.1038/s41467-022-28096-z

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.