KLPGWSG peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

• KLPGWSG polypeptide is a novel bioactive peptide with significant potential in neural tissue engineering. It was identified through Phage Display technique on murine neural stem cells (NSC) and was found to be present in proteins involved in stem cell maintenance and differentiation.
• This peptide binds to molecules on the cell surface of NSC and has been shown to enhance neuronal differentiation of NSC when added to the differentiation medium or linked to a self-assembling peptide (SAP) scaffold. When conjugated to a FITC fluorochrome, it can potentially be used as an NSC marker. The peptide does not substitute for or increase the proliferative effect of epidermal growth factor (EGF) on NSC and does not prevent cell death in the absence of EGF and basic fibroblast growth factor (bFGF). However, it positively modulates the neuronal differentiation of NSC, making it a promising candidate for biomimetic studies and stem cell therapies aimed at nervous system regeneration.
• The KLPGWSG-functionalized SAP scaffolds, such as Ac-KLP, with appropriate stiffness, support cell attachment and survival, and show enhanced neuronal differentiation compared to non-functionalized scaffolds, suggesting a synergistic effect of the peptide's presence and scaffold properties.

• Caprini A, Silva D, Zanoni I, Cunha C, Volontè C, Vescovi A, Gelain F. A novel bioactive peptide: assessing its activity over murine neural stem cells and its potential for neural tissue engineering. N Biotechnol. 2013 Jun 25;30(5):552-62. doi: 10.1016/j.nbt.2013.03.005. Epub 2013 Mar 26. PMID: 23541699.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.