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Product Name
Human KLK11/Kallikrein 11 (His Tag) recombinant protein
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Description
Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
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Protein name
Kallikrein-11
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Protein short names
MGC33060; MGC144345; KALLIKREIN 11; 2310015I08RIK; PRSS20; KLK11; TLSP
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Uniprot ID
Q9UBX7
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Gene Name
KLK11; PRSS20; TLSP; UNQ649/PRO1279
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human KLK11 isoform 1 (NP_006844.1) (Met 1-Asn 250) with a C-terminal polyhistidine tag was expressed.
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Protein Species
Human
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Molecular weight
The secreted recombinant human KLKL11 comprises 243 amino acids with a predicted molecular mass of 27 kDa. As a result of glycosylation, rhKLK11 migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468). The specific activity is >200 pmoles/min/μg.
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Validations
Human KLK11 / Kallikrein-11 Protein (His Tag) SDS-PAGE
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