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Product Name
ITPR1 Polyclonal Antibody
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Description
Polyclonal antibody to ITPR1
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ITPR1 antibody; ACV antibody; CLA4 antibody; INSP3R1 antibody; IP3R antibody; IP3R1 antibody; PPP1R94 antibody; SCA15 antibody; SCA16 antibody; SCA29 antibody; inositol 1,4,5-trisphosphate receptor type 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1250 of human ITPR1 (NP_001161744.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - ITPR1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ITPR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 10s.
Immunofluorescence - ITPR1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using ITPR1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"