Isotocin, (Ser4,Ile8)-Oxytocin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Isotocin and (Ser4,Ile8)-Oxytocin are neurohypophysial nonapeptides. Isotocin, found in many teleost fish, is structurally analogous to mammalian oxytocin, differing by a single amino acid substitution at position 4. (Ser4,Ile8)-Oxytocin is a synthetic analogue of oxytocin featuring specific substitutions at residues 4 and 8. These modifications alter the peptide's conformational and physicochemical properties compared to the native hormone.

The primary structure of these peptides allows them to interact with specific receptor subtypes. Research on such analogues, including Isotocin and (Ser4,Ile8)-Oxytocin, facilitates the study of ligand-receptor interaction mechanisms and structure-activity relationships. Investigations often employ techniques like peptide library screening and affinity binding assays to characterize their binding specificities and biophysical behaviors.

• Birnbaum S, Mosbach K. Peptide screening. Curr Opin Biotechnol. 1992 Feb;3(1):49-54. doi: 10.1016/0958-1669(92)90125-3. PMID: 1368024.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.